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Image Search Results
Journal: Frontiers in Cellular and Infection Microbiology
Article Title: Bioactivity of Farnesyltransferase Inhibitors Against Entamoeba histolytica and Schistosoma mansoni
doi: 10.3389/fcimb.2019.00180
Figure Lengend Snippet: Effect of tipifarnib on invasion of matrigel by E. histolytica . E. histolytica trophozoites were incubated for 3 h with 0.5% DMSO, 14 μM of tipifarnib and 25 μM of tipifarnib and at the end of incubation trophozoites were allowed to invade the matrigel for 48 h. After 48 h, the migrated cells in the lower chamber were counted and percentage invasion of trophozoites was calculated and plotted (A) . Images of trophozoites at 48 h were captured using a Zeiss Axiovert A1 inverted microscope and a Zeiss AxioCam 503 mono digital camera controlled by Zen 2 lite software. Trophozoites were treated for 3 h with 0.5% DMSO (B) , 14 μM tipifarnib (C) , and 25 μM tipifarnib (D) and migrated trophozoites in lower chambers were imaged at 48 h. Trophozoites were photographed at 10 × magnification. Arrow indicates E. histolytica trophozoites. ** P < 0.01 and *** P < 0.001 by Student's t -test compared to 0.5% DMSO-treated E. histolytica trophozoites.
Article Snippet: Images were captured via a Zeiss AxioCam 105 color digital camera that was attached to a
Techniques: Incubation, Inverted Microscopy, Software
Journal: Frontiers in Cellular and Infection Microbiology
Article Title: Bioactivity of Farnesyltransferase Inhibitors Against Entamoeba histolytica and Schistosoma mansoni
doi: 10.3389/fcimb.2019.00180
Figure Lengend Snippet: Phenotypic changes in E. histolytica upon administration of FTI lonafarnib in combination with metronidazole. E. histolytica trophozoites were photographed 24 h after treatment with lonafarnib and metronidazole using a Zeiss Axiovert A1 inverted microscope and a Zeiss AxioCam 503 mono digital camera controlled by Zen 2 lite software. The trophozoites were treated with 7.4 μM of lonafarnib alone (A) , 1.9 μM of metronidazole alone (B) , a combination of 7.4 μM of lonafarnib and 1.9 μM of metronidazole (C) , 0.5% DMSO (D) and 50 μM of metronidazole (E) . White arrow indicates normal morphology of trophozoites and black arrow indicates rounded, lysed trophozoites. Trophozoites were photographed at 20 × magnification.
Article Snippet: Images were captured via a Zeiss AxioCam 105 color digital camera that was attached to a
Techniques: Inverted Microscopy, Software